Recent advances in imaging technologies have revolutionized modern biology and medicine by providing researchers and health professionals with access to large amounts of image data at unprecedented levels of detail. Research at Duke has focused on the development of advanced computational tools for mining big data to extract patterns, for example, to elucidate the high-resolution structure of biomolecules under physiologically relevant conditions. This information can help biologists and physicians unveil the molecular mechanisms that regulate biological processes, providing more opportunities for the development of new medical treatments to combat disease.